BCodePro™ Fast Running Solution

Fast Electrophoresis with High Speed!

The speed of electrophoresis is closely related to its ionic strength.
BCodePro™ Fast Running Solution has a higher ionic strength compared to the conventional Tris-Glycine system, allowing more current flow and stable electrophoresis even under high voltage (up to 250 V).

In addition, it is well known that Tricine has a lower pKa than Glycine, resulting in a higher proportion of anionic forms and faster migration in the gel.
BCodePro™ Fast Running Solution uses a zwitter ion with electrophoretic mobility similar to Tricine, which accelerates the movement of charged species and promotes overall current flow during electrophoresis.

As a result:

• Conventional Tris-Glycine-SDS buffer requires about 60 min at 150 V
• While BCodePro™ Fast Running Solution completes electrophoresis in about 30 min at 150 V
• And in as little as 20 min at 250 V with a mini-size gel.

Clear Separation of Low-Molecular-Weight Proteins

BCodePro™ Fast Running Solution enhances the resolution of low-molecular-weight proteins by applying a principle similar to Tricine-SDS-PAGE. It contains a zwitter ion with electrophoretic mobility similar to SDS, improving the separation of low-molecular-weight proteins.

In Tricine-based systems, Tricine competes with low-molecular-weight proteins because its mobility is similar to that of SDS. This prevents them from migrating at the same rate as SDS micelles, resulting in their separation from SDS within the gel and producing sharp, high-resolution bands. Likewise, BCodePro™ Fast Running Solution uses a zwitter ion with SDS-like mobility, which reduces the migration speed of low-molecular-weight proteins.

As a result, when used with an 8% gel, it enables high-resolution separation of low-molecular-weight proteins even without a gradient gel, producing gradient gel-like electrophoretic patterns.

Does the Same Gradient Gel Effect Appear in Precast Gels?

In the conventional Tris-Glycine system, at an alkaline pH (~8.3), SDS–protein complexes carry a high negative charge, resulting in strong electrophoretic mobility under the electric field.
In contrast, at near-neutral pH (pH 6.4–7.0), SDS ionization is limited, leading to a relatively lower charge-to-mass ratio. This particularly slows the migration of low-molecular-weight proteins, thereby reducing resolution.

Most precast gels are formulated at near-neutral pH, and fixed gels (single acrylamide concentration rather than gradient gels) have uniform pore sizes. Consequently, the gradient gel-like effect generated by BCodePro™ Fast Running Solution is less pronounced in precast gels, as low-molecular-weight proteins pass through the gel more quickly, resulting in lower resolution compared to alkaline gels.

However, the high-speed electrophoresis effect remains the same in precast gels. Stable high-voltage electrophoresis (up to 250 V) can be achieved within 20 minutes even with precast gels.